Fig. 1

Trp53 gene deletion. After the design and synthesis of two highly active TALENs specific to exon 2 of Trp53, each TALEN mRNA was injected into the cytoplasm of mouse pronuclear-stage embryos to produce FVB/N-Trp53^em2Hwl/Korl (A) and C57BL/6-Trp53^em1Hwl/Korl (B) KO mice deficient in p53