Fig. 1
Myeloid-specific Hif-1α knockout mice exhibit an anti-obese phenotype. A Body weight changes in WT or myeloid-specific Hif-1α knockout (KO; hMRP8cre+; Hif-1αfl/fl; ApoE−/−) mice upon HFD. *** indicates P < 0.001 by two-way ANOVA. B Photographs of WT and myeloid-specific Hif-1α KO mice after 20 wk of HFD. C Photographs of isolated eWAT. D Quantification of the visceral (left) and subcutaneous (right) fat mass obtained from mice in (B) normalized to their body weight. ** and *** indicate P < 0.01 and 0.001, respectively, by Student’s t-test. E Femur lengths of mice in (B). F, G Daily food (left) and water (right) consumption (F) or daily feces (left) and urine (right) excretion (G) in setting of (B). Data are the mean ± s.e.m. from at least 5 animals per group. H Respiratory exchange ratio (RER) determined as VCO2/VO2 from treadmill exercise of WT or myeloid-specific Hif-1α KO mice after 20 wk of HFD. I Fasting blood glucose level in mice fed with NCD (n = 5 for WT mice; n = 6 for myeloid-specific Hif-1α KO mice) or HFD (n = 6 for WT mice; n = 6 for myeloid-specific Hif-1α KO− mice). *** indicates P < 0.001 by two-way ANOVA. J Glucose tolerance test in WT (n = 4) or myeloid-specific Hif-1α KO mice (n = 10) after 12wk of HFD. *, **, and *** indicate P < 0.05, 0.01, and 0.001, respectively, by two-way ANOVA. Symbols and error bars are the mean ± S.E.M