Fig. 2

Increased lipolysis and thermotolerance in myeloid-specific Hif-1α KO mice fed with HFD. A Representative hematoxylin and eosin stained sections (left) and the number of adipocytes counted per high power field (HPF; right) of the eWAT from WT or myeloid-specific Hif-1α KO mice fed with HFD for 12 wk. B Triglyceride (TG) levels in eWAT (left) or in plasma (right) of WT or myeloid-specific Hif-1α KO mice in (A). Mice were fasted for 8 h prior to the sample collection. * indicates P < 0.05 by Student’s t-test. C Gene expression changes in eWAT of mice in setting of (A). * indicates an increased gene expression change by more than twofold. D, E Western blot analyses for phosphorylated HSL (pHSL), HSL and/or UCP1 in WAT (D) or adipocytes (E) derived from WAT of WT or myeloid-specific Hif-1α KO mice after 12 wk of NCD or HFD. Actin was used as a loading control. F Core body temperature of WT (n = 6) or myeloid-specific Hif-1α KO mice (n = 5) fed with HFD upon cold challenge for 5 h. *, ** and *** indicate P < 0.05, 0.01 and 0.001, respectively, by two-way ANOVA. G The core body temperature of myeloid-specific Hif-1α KO mice (n = 2), WT mice surgically removed iWAT (n = 4) or myeloid-specific Hif-1α KO mice surgically removed for iWAT (n = 5) that had been fed with HFD for 12 wk upon cold change as in (F). Mice were allowed to recover 2 wk following the surgery before fed with HFD. Symbols and error bars are the mean ± S.E.M