Fig. 5

Increased acetyl-CoA in ATM deficient for HIF-1α may be responsible for the increased heat production. A Gene ontology (GO) analysis in the biological process of RNA sequencing results using FACS sorted ATM from WT or myeloid-specific Hif-1α KO mice fed with HFD for 12 wk. B Heat map of individual gene expression from RNA sequencing results in (A). C Acetyl-CoA production from the sorted ATM of WT or myeloid-specific Hif-1α KO mice fed with HFD. D mRNA levels of genes in FACS sorted ATM from WT or myeloid-specific Hif-1α KO mice fed with HFD. E Acetyl-CoA production from WT BMDM treated with vehicle or sodium acetate (5 mM). F Fold changes in acetyl-CoA levels in BMDM of WT or myeloid-specific Hif-1α KO mice treated with sodium acetate compared to those with the vehicle control. G ERthermAC (red) staining of WT BMDM treated with vehicle or sodium acetate. Nuclei are shown in blue with DAPI counterstaining. Scale bar denotes 50 μm. Quantification of ERthermAC intensity is shown on the right. *, ** and *** indicate P < 0.05, 0.01 and 0.001, respectively, by Student’s t-test. H ERthermAC (red) staining of BMDM transfected with empty, UCP1 OE, or UCP1 siRNA (KD) vector treated with or without sodium acetate. Nuclei are shown in blue with DAPI counterstaining. Scale bar denotes 100 μm. Quantification of ERthermAC intensity is shown on the right. *** indicate P < 0.001 by one-way ANOVA. Symbols and error bars are determined with the mean ± S.E.M