Fig. 2

The STAT1/IRF1 inhibitor Fluda attenuates LPS-induced ALI in mice. A pSTAT1 (Tyr701), pSTAT1 (Ser727), STAT1, IRF1, and β-actin were detected in mouse lung tissue. B Densitometric ratios of pSTAT1 (Tyr701) and pSTAT1 (Ser727) against STAT1 and that of IRF1 against β-actin. C pSTAT1 (Tyr701), pSTAT1 (Ser727), STAT1, IRF1, and β-actin expression was detected in BAL cell lysates using western blotting. D Densitometric ratios of pSTAT1 (Tyr701) and pSTAT1 (Ser727) against STAT1 and that of IRF1 against β-actin (n = 3). E Representative images of hematoxylin and eosin-stained lung sections from four experimental groups (× 200). Scale bar, 50 μm. F BAL cells were subjected to Giemsa staining and then observed under a microscope (× 200). Scale bar, 50 μm. G The lung injury score illustrated Fluda reduced LPS-induced ALI in mice (n = 4 per group). H The numbers of macrophages and neutrophils in BALF. I Total protein in BALF was measured using the BCA assay. J MPO activity was measured in whole-lung lysates. K Inflammatory cytokines in BALF, including TNF-α, IL-6, IFN-γ, and IL-1β, were detected using ELISA. The data are presented as mean ± SD, n = 3 independent experiments were performed. *p < 0.05, **p < 0.01, and ***p < 0.001 compared with the control group. †p < 0.05, ††p < 0.01, and †††p < 0.001 compared with the LPS group